Lupoi, J.S., S. Singh, M. Davis, D.J. Lee, M. Shepherd, B.A. Simmons, R.J. Henry, “High-Throughput Prediction of Eucalypt Lignin Syringyl/Guaiacyl Content Using Multivariate Analysis: a Comparison Between Mid-Infrared, Near-Infrared, and Raman Spectroscopies for Model Development,” Biotechnology for Biofuels, 7(1) 93, June 2014. DOI: 10.1186/1754-6834-7-93
In order to rapidly and efficiently screen potential biofuel feedstock candidates for quintessential traits, robust high-throughput analytical techniques must be developed and honed. The traditional methods of measuring lignin syringyl/guaiacyl (S/G) ratio can be laborious, involve hazardous reagents, and/or be destructive. Vibrational spectroscopy can furnish high-throughput instrumentation without the limitations of the traditional techniques. Spectral data from mid-infrared, near-infrared, and Raman spectroscopies was combined with S/G ratios, obtained using pyrolysis molecular beam mass spectrometry, from 245 different eucalypt and Acacia trees across 17 species. Iterations of spectral processing allowed the assembly of robust predictive models using partial least squares (PLS).
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