Jason S. Lupoi, Ph.D.
Consulting Scientist & Analytical Chemist
Before coming to RJ Lee Group, Dr. Lupoi led research projects predominantly focused on the creation of multivariate analysis data models. These models were developed using vibrational spectroscopy coupled with reference methods, such as chromatography that enabled the use of high-throughput spectroscopic instrumentation for the rapid screening of a diverse array of plants.
This research included the marriage of FTIR, NIR and FT-Raman spectral data with pyrolysis molecular beam mass spectrometry to create partial least squares data models for predicting eucalypt syringyl/guaiacyl content, as well as evaluate which type of vibrational spectroscopy led to the most robust multivariate models. Dr. Lupoi’s other work includes: the creation of a novel infrared imaging method that compared wild-type and modified sugarcane for localizing polyhydroxybutyrate expressed in sugarcane, and a NIR Raman spectroscopic method for characterizing lignocellulosic feedstocks with emphasis on lignin monomer and extractable content that enables plants to be analyzed rapidly, and non-destructively in situ. This method coupled NIR Raman spectroscopy and thioacidolysis/GCMS to construct principal component analysis and principal component regression models for predicting the lignin monomer composition, and a technique for immobilizing cellulase to silica nanoparticles for use in simultaneous saccharification and fermentation reactions that led to a two-fold increase in ethanol concentration.
Dr. Lupoi has authored several peer-reviewed manuscripts, review articles, and a book chapter, has been an associate editor of a Special Topic journal in Frontiers in Biotechnology and Bioengineering, and has been a reviewer for several leading biofuel and analytical chemistry journals.