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Ambient particulate matter (PM) is a complex mixture that includes bioactive and toxic compounds of natural and anthropogenic origin, several of which have been theorized to cause or at least contribute to adverse health effects from inhalation. The U.S. Environmental Protection Agency (EPA) initiated an experimental assessment of the health effects from exposure to coarse, fine and ultra-fine (UF) fractions corresponding to particles with mean aerodynamic diameters of >2.5, >0.1-2.5, and <0.1 micrometers, respectively. A key component of this study is the evaluation of healthy adult subjects exposed to PM fractions in specially designed aerosol chambers. Determination of the size distributions and composition of the PM introduced to the chambers is performed with real time measurements and microscopy analysis.
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