Capabilities

We provide testing services to certify product and materials compliance, support quality control, and ensure the health and safety of workers and their environment. We provide assistance for clients who are not sure what tests they need. Our accredited materials characterization laboratory is the foundation of all the services of RJ Lee Group provides, including standardized testing for compliance, industrial hygiene and environmental analyses, quality control, and materials research and development.

We provide credentialed expertise supported built by robust scientific data. We provide significant support to industrial clients for product development, industrial hygiene and overall production support.

RJ Lee Group maintains a visible and respected reputation within the legal community, having offered scientific support in civil litigation matters for nearly 30 years. 

  • Product Liability 
  • Toxic Tort
  • Construction Defect
  • Fugitive Emission
  • Personal Injury
  • Patent Infringement
  • Contract Disputes
  • Insurance Subrogation

Subject Matter Experts

From our core business of providing scientific solutions to our clients, we have developed innovative products. Some are produced internally, and some have arisen from partnerships with other research organizations. 

For example, we build lab software solutions to help manage and streamline your labs data, and environmental testing products for a variety of applications. 

  • IntelliSEM is a powerful automated particle analysis system.
  • ParticleID is  a customizable cloud hosted web application used to identify foreign particulate matter and  assist with root cause investigations. 

Other products we create count particles and help keep the air and environment safe.

RJ Lee Group is a materials analysis laboratory and consulting company which serves many different industries. We offer scientific solutions such as industrial forensics services, laboratory and testing services, litigation support, and laboratory software to many industries:

Application of the Liquid Chromatography/Mass Spectrometer/Mass Spectrometer (LC/MS/MS) Instrument

RJ Lee Group News

July 12, 2018

LC/MS/MS Instrument Arrives at RJLG

Recently obtained by the RJ Lee Group, the liquid chromatography/mass spectrometer/mass spectrometer (LC/MS/MS) is an instrument with exceptional capability for both qualitative and quantitative analysis. It has remarkable sensitivity and is capable of providing fast and accurate identification of targeted compounds of interest.

In many instances the instrument will be able to provide the following:
• Lower reporting limits
• Greater specificity
• Faster analysis times
• Simplify sample preparation procedures
• Improved problem solving capabilities

Mass Analyzers

This instrument takes advantage of two separate mass analyzer designs.

The first is the triple quadrupole configuration, which supports eight different types of mass spectrometry experiments. There are three quadrupole sections in the instrument. In some modes of operation, the first quadrupole (Q1) permits the operator to select a specific ion for a compound. The second quadrupole (Q2) is used for fragmentation of that selected ion. The third quadrupole (Q3) can either be used to monitor a specific ion from the second fragmentation process, or to collect the entire mass spectrum resulting from the Q2 fragmentation of the Q1 selected ion. One can imagine several experiments that can be used for qualitative and quantitative analysis. Among these is the ability to perform multiple reaction monitoring (MRM) measurements for quantitative determinations with exceptionally low reporting limits.

The second mass analyzer design simply changes the electronic configuration of Q3 to make it function as a linear ion trap (LIT). Conventional ion trap systems are plagued by space charge effects. These are caused when the electrons emitted by the cathode are repulsed by the cloud of electrons near the cathode itself. The maximum ion density in the trap must be controlled and limited in order to reduce this occurrence. This will decrease premature ejection of the ions and the loss of mass resolution. The LIT permits the trapped ion density to increase significantly, causing substantial increases in sensitivity. Six additional mass spectrometry related experiments can be performed when the instrument is operated in the LIT mode.

Ionization

The LC/MS/MS only works if a target compound can be ionized before it is introduced to the instrument. The ionization processes most commonly used are electrospray (ESI), or one if its variants, and atmospheric pressure chemical ionization (APCI). Both are readily used for samples introduced by a liquid chromatograph (LC) or direct liquid infusion (DLI). Both are ionization processes that occur at atmospheric pressure rather than in a vacuum as in conventional gas chromatography/mass spectrometry (GC/MS) using electron ionization (EI).

Gas chromatography, another method used for identifying compounds, requires a target compound to have some degree of volatility, and to be stable under elevated thermal conditions during the analysis process. This limits the use of GC to the analysis of less than 3% of the known chemicals in the Chemical Abstracts Registry. Liquid chromatography (LC), conversely, is potentially amenable to any compound that can be dissolved in an aqueous or organic solvent. Liquid Chromatography consequently becomes a powerful separation and sample introduction technique to a mass spectrometer. This enables the analysis of a broad range analytes, and subsequently, a broad range of applications.

Instrument Applications

Applications of LC/MS/MS are extensive. A good resource for applications can be found in the vendor catalogs of the LC columns. Many of these vendors produce chromatograms of families of compounds such as nitrosamines, pesticides, drugs of abuse, pharmaceutical byproducts, carbohydrates, antibiotics, aflatoxins, peptides and proteins, explosives, aldehydes and ketones, to name a few.

A successful analysis depends in part in isolating the target analytes from the sample matrix and performing the chromatographic separation. In many cases, sample cleanup can be simplified by taking advantage of the power of the instrument to perform selective analysis of the ion(s) that indicate the presence of the target analyte(s) by using the MS/MS function (a mass spectrometer within a mass spectrometer). The analytical technique can be applied to samples originating from industrial hygiene monitoring, environmental sampling, fermentation broths, growth media, agriculture industry, process control, biomedical analyses, and countless others.

For more information on the LC/MS/MS, please click the button below or call (509.545.4989).

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